Sankalp Nanda – Unlocking the Secrets of Molecular Genetics: A Career Ready to Take Off in Biopharma!

PROFESSIONAL SUMMARY

MSc graduate specializing in Molecular and Human Genetics with hands-on experience in mammalian cell culture, flow cytometry, and omics data analysis. Seeking an industry role in biopharma R&D, quality control, or product development where I can contribute to translational research and pipeline development in a GMP-regulated environment.

TECHNICAL SKILLS

Core Lab Techniques

• Mammalian Cell Culture: HeLa, THP1, Caski, SIHA, C33a, U937, HIF1α knockdown, RAW264.7, MSC
• Media Prep: DMEM, RPMI1640, aseptic handling and passaging
• Protein and Nucleic acid techniques: PCR, SDS-PAGE, ELISA, Western Blot, BCA/Bradford assay
• FACS: BD FACSVerse, BD Influx; FloJo for analysis
• Sample Handling: PBMC and PRP isolation, Animal dissection (BALB/c, C57BL6), Blood collection

Omics and Bioinformatics

• Metabolomics: Progenesis QI, Metaboanalyst
• Gene Interaction: STRING Database
• Primer Design and Alignment: BLAST, Primer3, ClustalW

Lab Standards and Safety

• BSL3 compliance, GLP-compliant workflow execution, Sample tracking, SOP compliance & writing, Biosafety, and Hazard handling, record keeping and Audit readiness

Soft Skills

• Analytical thinking, Time Management, Scientific Documentation, Teamwork, Self-Motivation

WORK EXPERIENCE

Career Break

Jan 2022 – May 2025

• Took a planned break to prepare for UPSC Civil Services Examination
• Developed advanced critical reasoning, discipline, and structured learning habits
• Now fully committed to transition back and actively pursuing roles in Biotech/Pharma and clinical data domains to apply Scientific skills in regulated, team-based environments

Junior Research Fellow (PhD Scholar)

UNESCO- Regional Centre for Biotechnology, Faridabad (Nov 2020 – Dec 2021)

Projects:

• Comparative Metabolomic and Transcriptomic profiling of Tibetan Highlanders (Native vs Migrant)
• SARS-COV2 immuno-phenotyping using Patient samples

• Conducted experiments in a GLP-compliant sterile lab and BSL3 facility
• Maintained mammalian cell cultures and processed human blood samples
• Performed immune cell profiling using FACS and ELISA
• Metabolomics data interpretation using Progenesis QI and Metaboanalyst
• Documented protocols and experiment logs as per compliance guidelines

Research Intern

Department of Biotechnology, JNU, New Delhi (May 2019 – Jul 2019)

• Performed murine bone marrow extraction and cultured mesenchymal stem cells(MSCs)
• Conducted infection of MSCs with Mycobacterium tuberculosis in a BSL3 setting

ACADEMIC PROJECTS

MSc Thesis- BHU, Varanasi

“Effects of MG132 on various Cervical Cancer Cell Lines”

• Assessed apoptotic effects of proteasome inhibitor MG132 on HeLa, Caski, and SiHa cells
• Performed MTT assays, Western blotting, and protein estimation (Bradford assay)
• Relevance: Drug sensitivity profiling for anticancer therapy

BSc Thesis- IMR-RMRC, Bhubaneswar

“Effects of Smoking on Human Oral Microbiota”

• Collected and cultured microbial swabs from Human subjects
• Studied changed in oral microbiota diversity due to tobacco exposure

EDUCATION

MSc Molecular and Human Genetics

BANARAS HINDU UNIVERSITY, Varanasi (Jun 2018 – Jun 2020 | CGPA- 8.24)

BSc(Hons) Zoology

BJB (Autonomous) College, UTKAL UNIVERSITY (07/2015 – 05/2018 | CGPA: 8.36)

CERTIFICTIONS (In progress/Planned)

• Good Laboratory Practices(GLP)
• Good Manufacturing Practices (GMP)
• Regulatory Affairs Essentials
• ICH-GCP for Clinical Trials
• Clinical Data Management

CONFERENCES AND WORKSHOPS

• 22nd INDO-US Flow Cytometry Workshop – TECT & ISAC (2021)
• Odisha State Biotechnology Ignition Challenge(OSBIC) – 2nd rank in Biotech quiz
• Programme on Young Graduates for Leadership in Biotech(PYGLB) workshop- SOA university (2018)
• 35th Annual Conference of Indian Society of Neuroscience, Ravenshaw University (2017)

REFERENCES

Available on request.

Skills/Keywords

Molecular and Human Genetics, Mammalian Cell Culture, Flow Cytometry, Omics Data Analysis, GLP, BSL3, FACS, ELISA, Metabolomics, Transcriptomics, Protein Techniques, Nucleic Acid Techniques, Lab Standards, GMP, Regulatory Affairs, Bioinformatics, Teamwork, Time Management, Analytical Thinking, Scientific Documentation, PCR, Western Blot, Cell Culture, Dissection

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Cover Letter:

I am writing to express my keen interest in the position of Microbiology Executive at Kwality Pharmaceuticals Ltd. While I may not have extensive direct work experience in microbiology, my academic foundation and strong laboratory background in molecular biology, aseptic techniques, and GLP-compliant operations have prepared me to effectively contribute to your quality control and product testing initiatives.
During my Master’s and subsequent research tenure at RCB (under UNESCO), I gained hands-on experience with sterile technique, mammalian and microbial cell cultures, ELISA, PCR, and flow cytometry. My undergraduate thesis specifically explored the effects of smoking on oral microbiota, involving the collection, culturing, and analysis of human microbial swabs—an experience that introduced me to core microbiological techniques. Additionally, I’ve worked in BSL3 laboratories, handling live pathogens (Mycobacterium tuberculosis) and processed clinical samples under stringent biosafety conditions, which aligns well with the regulatory rigor expected in your microbiology labs.
What particularly draws me to Kwality Pharmaceuticals is your remarkable growth in regulated markets and diverse product portfolio across critical dosage forms. I am motivated to grow with an organization that values quality, innovation, and scientific precision. I am currently upskilling in GMP, GLP, and Regulatory Affairs to complement my academic experience with industry best practices.
I bring with me a mindset of structured problem-solving, meticulous documentation, and teamwork, along with a deep desire to return to the pharmaceutical domain after a brief academic sabbatical. I am confident in my ability to adapt quickly, learn from your team, and contribute meaningfully to your microbiological and quality assurance efforts.
Thank you for considering my application. I look forward to the opportunity to further discuss how my background can support your goals at Kwality Pharmaceuticals.
Warm regards,
Sankalp Nanda