SANKALP NANDA
Research Associate
Bhubaneswar, India
PROFESSIONAL SUMMARY
MSc graduate specializing in Molecular and Human Genetics with hands-on experience in mammalian cell culture, flow cytometry and omics data analysis. Seeking an industry role in biopharma R&D, quality control, or product development where I can contribute to translational research and pipeline development in a GMP-regulated environment.
TECHNICAL SKILLS
Core Lab Techniques
• Mammalian Cell Culture: HeLa, THP1, Caski, SIHA, C33a, U937, HIF1α knockdown, RAW264.7, MSC
• Media Prep: DMEM, RPMI1640, aseptic handling and passaging.
• Protein and Nucleic acid techniques: PCR, SDS-PAGE, ELISA, Western Blot, BCA/Bradford assay
• FACS: BD FACSVerse, BD Influx; FloJo for analysis.
• Sample Handling: PBMC and PRP isolation, Animal dissection (BALB/c, C57BL6), Blood collection
Omics and Bioinformatics
• Metabolomics: Progenesis QI, Metaboanalyst
• Gene Interaction: STRING Database
• Primer Design and Alignment: BLAST, Primer3, ClustalW
Lab Standards and Safety
• BSL3 compliance, GLP- compliant workflow execution, Sample tracking, SOP compliance & writing, Biosafety and Hazard handling, record keeping and Audit readiness
Soft Skills
• Analytical thinking, Time Management, Scientific Documentation, Teamwork, Self-Motivation
WORK EXPERIENCE
Career Break
Jan 2022 – May 2025
• Took a planned break to prepare for UPSC Civil Services Examination
• Developed advanced critical reasoning, discipline, and structured learning habits.
• Now fully committed to transition back and actively pursuing roles in Biotech/Pharma and clinical data domains to apply Scientific skills in regulated, team-based environments.
Junior Research Fellow (PhD Scholar)
UNESCO- Regional Centre for Biotechnology, Faridabad
Nov 2020 – Dec 2021
Projects:
1) Comparative Metabolomic and Transcriptomic profiling of Tibetan Highlanders (Native vs Migrant).
2) SARS-COV2 immuno-phenotyping using Patient samples.
• Conducted experiments in a GLP-compliant sterile lab and BSL3 facility.
• Maintained mammalian cell cultures and processed human blood samples
• Performed immune cell profiling using FACS and ELISA
• Metabolomics data interpretation using Progenesis QI and Metaboanalyst.
• Documented protocols and experiment logs as per compliance guidelines.
Research Intern
Department of Biotechnology, JNU, New Delhi
May 2019 – Jul 2019
• Performed murine bone marrow extraction and cultured mesenchymal stem cells(MSCs)
• Conducted infection of MSCs with Mycobacterium tuberculosis in a BSL3 setting
ACADEMIC PROJECTS
MSc Thesis- BHU, Varanasi
“Effects of MG132 on various Cervical Cancer Cell Lines”
• Assessed apoptotic effects of proteasome inhibitor MG132 on HeLa, Caski, and SiHa cells
• Performed MTT assays, Western blotting, and protein estimation (Bradford assay)
• Relevance: Drug sensitivity profiling for anticancer therapy
BSc Thesis- IMR-RMRC, Bhubaneswar
“Effects of Smoking on Human Oral Microbiota”
• Collected and cultured microbial swabs from Human subjects
• Studied changes in oral microbiota diversity due to tobacco exposure
EDUCATION
MSc Molecular and Human Genetics
BANARAS HINDU UNIVERSITY, Varanasi
Jun 2018 – Jun 2020 | CGPA- 8.24(Rank 2 out of 26)
Key Courses- Cancer Genomics, Pharmacogenomics, Biostatistics, Immunogenetics, Bioinstrumentation
BSc(Hons) Zoology
BJB (Autonomous) College, UTKAL UNIVERSITY
07/2015 – 05/2018 | CGPA: 8.36
Competitive Exams (Qualified):
CSIR NET JRF (AIR43- Jun 2019, AIR61- Dec 2019), GATE (AIR51- 2020, BL paper)
CERTIFICTIONS (In progress/Planned)
• Good Laboratory Practices(GLP)
• Good Manufacturing Practices (GMP)
• Regulatory Affairs Essentials
• ICH-GCP for Clinical Trials
• Clinical Data Management
CONFERENCES AND WORKSHOPS
• 22nd INDO-US Flow Cytometry Workshop – TECT & ISAC (2021)
• Odisha State Biotechnology Ignition Challenge(OSBIC) – 2nd rank in Biotech quiz.
• Programme on Young Graduates for Leadership in Biotech(PYGLB) workshop- SOA university (2018)
• 35th Annual Conference of Indian Society of Neuroscience, Ravenshaw University (2017)
REFERENCES
Available on request.
Skills: Molecular and Human Genetics, Mammalian Cell Culture, Flow Cytometry, Omics Data Analysis, Metabolomics, Transcriptomics, GLP, GMP, Bioinformatics, Lab Safety, Data Interpretation, Teamwork, Time Management, Documentation
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Cover Letter:
I am writing to express my interest in the position of Bioanalytical Analyst at Skylimit Research Pvt. Ltd. With a postgraduate degree in Molecular and Human Genetics from Banaras Hindu University and hands-on experience in clinical sample handling, flow cytometry, ELISA, and omics data interpretation, I am excited by the opportunity to contribute to your expanding bioanalytical operations and support the high regulatory standards Skylimit is known for.
During my time as a Junior Research Fellow at UNESCO-Regional Centre for Biotechnology, I worked extensively in a GLP-compliant and BSL3 environment. I processed human blood samples, performed immunophenotyping using flow cytometry, and carried out cytokine quantification via ELISA. My work also involved the use of software platforms such as Progenesis QI and MetaboAnalyst for metabolomic data analysis, giving me exposure to quality control, data integrity (ALCOA+ principles), and regulatory documentation practices.
Skylimit’s reputation for cutting-edge infrastructure—featuring advanced LC-MS/MS instrumentation, robust quality systems, and full-spectrum bioanalytical services—makes it an ideal environment for a fresher like me to grow into a rigorous, compliance-driven analyst. I am currently upskilling with certifications in GLP, GMP, and ICH-GCP to strengthen my alignment with the standards expected in regulated bioanalytical workflows.
I am confident that my research experience, attention to detail, and passion for precision in biological data interpretation make me a strong fit for your team. I am particularly drawn to Skylimit’s vision of providing globally benchmarked CRO services and would be thrilled to contribute to your clinical and bioanalytical excellence.
Thank you for considering my application. I look forward to the opportunity to discuss how my skills and mindset can add value to Skylimit Research’s mission.
Warm regards,
Sankalp Nanda
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