ANKIT DHOUNDIYAL (Life Science Researcher)
Location: Dehradun, India
Profile Statement
Life science researcher specializing in cellular and molecular neuroscience, bioinformatics, scientific editing in silico drug discovery, mammalian cell culture, and image analysis.
Research Experience
Bioinformatics Researcher | Biotecnika, Remote | Jul 2024–Present
Project Title: In-silico drug discovery
- Screened 500+ phytochemicals from 8 different medicinal plants to identify potential inhibitors of key monkeypox virus proteins using ADMET profiling.
- Performed ligand–protein docking for the selected phytochemicals, aiming to identify potential drug candidates.
[hidden] Researcher | Medical University of Vienna | Jan 2021–Apr 2024
- Utilized live-cell fluorescence microscopy, patch-clamp electrophysiology, and pharmacological agents to investigate how voltage-gated calcium channels (VGCCs) regulate neuronal metabolism.
- Induced electrical activity in primary rat hippocampal neuronal cultures using electric field stimulation (EFS) to study the synaptic activity. Tracked intracellular Ca²⁺, ATP, and NADH using fluorescent markers.
Key Findings:
- Dissected the relative contribution of different Ca2+-stimulated pathways in neuronal ATP production.
- Investigated ER Ca2+ release and its effect on mitochondrial metabolic stimulation.
Researcher | National Brain Research Center | Jun 2019–May 2020
- Established baseline expression data of 3 different DNA-demethylating enzymes during the differentiation of neuronal stem cells using RT-PCR and western blotting, providing critical insights for future epigenetic studies.
- Analyzed baseline expression profile (mRNA and protein) of these 3 enzymes in 4 different mouse organs and neuroblastoma cell lines.
Education
[hidden] in Molecular Signal Transduction | Medical University of Vienna | Jan 2021–Apr 2024
Thesis project: Role of L-type calcium channels (LTCCs) in the regulation of neuronal mitochondrial metabolism.
[hidden] in Neuroscience | National Brain Research Centre, India | Aug 2018–Jun 2020
Thesis project: The role of epigenetic modifications during neuronal proliferation and differentiation.
[hidden] (Hons) in Botany | University of Delhi | Jul 2015–Jun 2018
Publications
- Dhoundiyal, A., Goeschl, V., Boehm, S., Kubista, H., & Hotka, M. Role of endoplasmic reticulum–mitochondria Ca2+ transfer in stimulating neuronal ATP production (in preparation).
- Dhoundiyal, A., Goeschl, V., Boehm, S., Kubista, H., & Hotka, M. (2022). Glycerol-3-phosphate shuttle is a backup system securing metabolic flexibility in neurons. The Journal of Neuroscience, JN-RM-0193.
- Kumar S., Mikawlrawng K., Dhoundiyal A. et. al. (2016). Ethnobotanical survey of antimicrobial flora of Manipur: A biodiversity hotspot region of North East India. Journal on New Biological Reports 5(3) 139-147.
Skills
Cell/Molecular biology, Microscopy, Bioinformatics, Technical proficiency, Programming Languages
Keywords: Mammalian cell culture, patch-clamp electrophysiology, RT-PCR, Western blotting, ligand–protein docking, MATLAB, R, Python, PubMed, immunocytochemistry, light microscopy, protein structure modeling, Microsoft Office, ImageJ
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Cover Letter:
Dear Hiring Team,
I would like to apply for the Genome Analyst position at MedGenome Labs Ltd. With a [hidden] in Molecular Signal Transduction from the Medical University of Vienna and a strong background in molecular biology, neurobiology, genetics, and bioinformatics, I am eager to contribute to your cutting-edge work in clinical genomics.
My doctoral research focused on calcium signaling and neuronal metabolism, employing advanced imaging and electrophysiological techniques. Additionally, I have hands-on experience with mammalian cell culture, RT-PCR, Western blotting, and fluorescence microscopy. More recently, I have been involved in an in-silico drug discovery project, performing ligand-protein docking and ADMET profiling, further strengthening my bioinformatics and data analysis skills. Although my experience doesn’t involve NGS sequencing yet, I will be happy to learn the desired tools and techniques with some internal guidance.
My experience in managing lab projects and conducting collaborative research has equipped me with the ability to collaborate well with your internal research projects and lab activities. Additionally, my work in bioinformatics—screening and profiling potential drug candidates—has given me experience in using virtual tools for data analysis, which would support tasks such as sequence analysis, primer design, and documentation.
In addition to my technical skills, I have demonstrated the ability to work effectively in team settings, manage multiple projects, and communicate complex concepts clearly. My role as a freelance Academic and English editor has further enhanced my organizational skills, attention to detail, and ability to work under tight deadlines.
Thank you for considering my application. I look forward to hearing from you.
Sincerely,
**Ankit Dhoundiyal**
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