Prafulla Srivastava

Profile

Dynamic and passionate biotechnologist with a strong foundation in both wet and dry lab practices. Proficient in handling advanced laboratory instruments and techniques, including SDS-PAGE, chromatography, plant tissue culture, and in silico gene characterization. Actively participated in esteemed national and international conferences, showcasing research aptitude and networking with global experts. Completed comprehensive training programs in biotechnology, enhancing technical and analytical skills. A well-rounded individual with leadership experience in cultural events, demonstrating exceptional teamwork and organizational abilities. Driven to excel in innovative biotechnological research and contribute to groundbreaking advancements.

Professional Experience

Project on Machine learning approach to find novel anti diabetic drug from natural sources

Biotecnika Info Labs Pvt Ltd
Role: Research Associate/Project Lead
Responsibilities:

Designed and implemented a data-driven workflow integrating machine learning models and bioinformatics tools for drug discovery.

Curated and pre-processed datasets of natural compounds, including their physicochemical properties, ADME profiles, and bioactivity data.

Extracted molecular descriptors such as molecular weight, LogP, and Ali Log S using cheminformatics tools like RDKit and SwissADME.

Developed and validated predictive machine learning models (Random Forest, Decision Tree, Linear Regression) to evaluate compound efficacy and drug-likeness.

Conducted statistical analysis and visualization of data using Python

Trainee at Experiome Biotech Private Limited

Completed training in biotechnology, acquiring expertise in wet lab techniques such as SDS-PAGE, chromatography, plant tissue culture, microbiology lab culture, and agarose gel electrophoresis. Proficient in dry lab practices like in silico gene family characterization. Skilled in laboratory protocols, data analysis, and molecular biology research methodologies.

Trainee at Biogrademy

Leaning basics of python and bio python libraries

Trainee at CytoGene Research & Development LLP

Training of basic bioinstrumentation tools like protein quantification assays, Agarose gel electrophoresis and DNA extraction from various sources

Student Coordinator at Apni Pathshala, SRMU

Part of CSR initiative of the University at apni pathshala runs for taking care of primary studies of the students of surrounding villages

Project dissertation at National centre for cell science

– Investigated the role of secretory PD-L1 (secPD-L1) in modulating T-cell exhaustion and apoptosis using Jurkat E6.1 cell line models
– Designed and optimized Western blot, pull-down assays, and co-immunoprecipitation to analyze PD-L1/PD-1 and FBXO family protein interactions
– Hands-on experience in protein concentration (cut-off 3kDa), SDS-PAGE, PVDF transfer, antibody probing, and chemiluminescence detection
– Performed ELISPOT/activation assays and used Jurkat + co-culture systems to validate secretory PD-L1’s functional role

Education

M.Sc. Biotechnology

Highly motivated MSc Biotechnology graduate with hands-on experience in advanced laboratory techniques, including column chromatography, centrifugation, SDS-PAGE, PCR, UV spectroscopy, and agarose gel electrophoresis. Proficient in handling laboratory equipment with precision and adhering to strict safety protocols. Actively participated in multiple national and international conferences, showcasing research work and engaging with global scientific communities. Demonstrates strong analytical skills, a commitment to innovation, and a passion for contributing to cutting-edge biotechnological advancements.

B.Sc. (Hons) Biotechnology

Shri Ramswaroop memorial University

Skills

• PCR
• Chromatography
• Gel electrophoresis
• HPLC
• Protein Purification
• Cell Proliferation Assays (MTT, CCK-8)
• Machine learning
• Cell cultures
• Python
• Molecular Cloning
• Immunoprecipitation (IP)
• Scientific Writing

Languages

• English
• Hindi

Certificates

• Volunteer at AMI international conference 2024 – Managed various national and international delegates as well as part of managing their accommodation and stay
• Best actor award at freshers Harmony 2022 at SRMU
• Coordinator at Shaswat fest at SRMU
• Certified in HPLC, GC and PCR handling
• Part of NSS Republic day parade
• Sports coordinator at Annual sports fest Ullas 2023 at SRMU
• Student coordinator at International Conference on Advances in Cancer Biology, Prevention & Therapy, SRMU 2022

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Cover Letter:

I can help with that. Please provide the data that needs to be processed.