Istekhar Alam – Unlocking the Secrets of Biotechnology: A Journey from Classroom to Cutting-Edge Research

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ISTEKHAR ALAM

Vilas Patil Building, Room No. 02, Navde, Navi Mumbai, Maharashtra – 410208

OBJECTIVE

Postgraduate in Biotechnology with hands-on experience in molecular biology techniques, including PCR, electrophoresis, cloning, and blotting. Backed by research training at BARC, I am seeking a challenging position in R&D or QC/QA where I can contribute to scientific innovation, ensure product quality, and support regulatory compliance through methodical analysis and attention to detail.

EDUCATION

  • Master of Science in Biotechnology: University of Mumbai, Kalina. 2023-2025 (CGPA 8.18)
  • Bachelors of Science in Biotechnology: Changu Kana Thakur College, Panvel. 2020-2023 (CGPA 8.49)

SKILLS & ABILITIES

RESEARCH

  • DNA Manipulation, PCR, Gel Electrophoresis, protein isolation, protein purification, Western Blotting, Southern Blotting, Plating, Culturing, RNA Isolation
  • Handling: Thermo Cycler, Sonicator, Nano Drop, Autoclave, PH Metre, Kingfisher Flex (RNA Extractor)

TOOLS

  • Microsoft Office (Word, Excel, PowerPoint)
  • Blast, Clustal W, Mega 11 (Bioinformatics)

COMMUNICATION

  • Good Communication Skill, Fluent English Speaking, Active listening, Team Collaboration

EXPERIENCE

  • BHABHA ATOMIC RESEARCH CENTRE (BARC)
  • (03/10/2024) (4 months)
  • Cloning of Putative Serine/Threonine Kinases of Radiation Resistant Bacterium Deinococcus radiodurans.
  • Conducted PCR amplification and gel electrophoresis to confirm successful gene cloning.
  • Performed protein expression using pET28a expression vector and purification using affinity chromatography to obtain target kinases.
  • LUPIN DIAGNOSTICS
  • (01/02/2024) (1 month)
  • Participated in on-the-job training program focused on molecular diagnostic techniques.
  • Gained practical experience in sample processing, PCR setup, and data analysis.
  • Developed skills in aseptic techniques, quality control procedures, and operation of automated analyzers.

I hereby declare that the information furnished above is true and correct to the best of my knowledge and belief.

SKILLS: Keywords: DNA Manipulation, PCR, Gel Electrophoresis, Western Blotting, RNA Isolation, Thermo Cycler, Microsoft Office, Bioinformatics, Good Communication Skill

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Cover Letter:

Dear Hiring Manager,

I am writing to express my keen interest in the Research Associate position at E-Merge Tech. With a Master’s degree in Biotechnology from the University of Mumbai and hands-on experience in molecular and cell biology, I am excited about the opportunity to contribute to your innovative research environment.
During my academic and research journey, I have worked extensively with techniques such as PCR, agarose and polyacrylamide gel electrophoresis, Western and Southern blotting, and protein/gene manipulation. My project at Bhabha Atomic Research Centre involved cloning a kinase and a phosphatase in Deinococcus radiodurans, strengthening my skills in microbial genetics and recombinant DNA technology.
I am particularly drawn to E-Merge Tech’s focus on leveraging advanced biotechnology for real-world impact. I admire your commitment to research-driven innovation, and I am confident that my background and drive align well with your goals. I am eager to bring my lab expertise, analytical mindset, and collaborative spirit to your team.

Thank you for considering my application. I look forward to the opportunity to discuss how I can contribute to E-Merge Tech’s research initiatives.

Sincerely,
Istekhar Safjal Alam

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